Peptidszintézis

PEPotec Immunpeptid könyvtárak

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Ideal for epitope mapping and high-throughput screening for immunology applications.

Thermo Scientific™ PEPotec™ Immuno Peptide Libraries are fully synthetic custom libraries that support high-throughput screening assays to map epitopes or identify immunogenic sites in proteins.

PEPotec Immuno Peptide Libraries are fully customizable and supplied with acetate as the counter ion to avoid potential toxicity issues in epitope discovery and mapping. These application-specific peptide libraries meet the requirements for almost instant use in applications such as vaccine development, T- and B-cell research, antibody development, and biomarker discovery. Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events.

Every peptide synthesis is a challenge. At Thermo Fisher Scientific, we have the experience, equipment and knowledge to meet your needs for custom peptide synthesis. Our synthesis team has accumulated tremendous expertise through producing tens of thousands of successful custom peptides. We are constantly adapting our product offering to your needs based on your input. Our expert peptide scientists will support you from the design phase of your peptide to choosing the right modifications, scale or purity for your assay so you achieve the best experimental results for your application.

Thermo Scientific™ Custom Peptide Capabilities:

• Peptides 6-40 amino acids (• Scales from 0.1mg - 1g • Purities from crude to 98%
• Extensive list of modifications and labels, including heavy peptides
• Flexible formatting options
• Custom conjugation services; e.g., protein-peptide conjugates and multiple antigen peptides (MAPS)

Synthesis

The choice of synthesis platform can affect the cost and/or success of a peptide synthesis. We use several platforms for fully automated FMOC solid-phase synthesis to provide state-of-the-art products. High-throughput synthesis platforms using 96-well reaction chambers offer longer length and higher purities than spot synthesis platforms. We also offer manual and semi-automated syntheses that usually are better choices for peptides with difficult sequence compositions.

Quality Control

All Thermo Scientific™ Peptides are synthesized in ISO 9001-certified facilities. Quality control for our standard peptide service includes mass spectrometry for identification and analytical HPLC for purity. The detailed spectra is included on the certificate of analysis for your records. Additional quality controls such as amino acid analysis (AAA) and further non-standard analysis are available on request.

Delivery Format

Unless otherwise stated, our standard peptides are delivered lyophilized with trifluoroacetate (TFA) as a counter ion and packaged in glass vials protected under argon. Exceptions are Thermo Scientific™ Heavy and Light AQUA Kits and our Peptide libraries that are provided in solution and argon-protected. For additional formats, such as delivery in low binding plastics or 2-D barcoded plates or in solution and different solvents, please inquire.

Interactiva Manufacturing Practice (IMP) Service

Our IMP service provides enhanced traceability and documentation to support requirements of customers who want to sell diagnostic services or apply for a certification for their products used in diagnostics.

Peptide Applications

The use of synthetic peptides has had a great impact on many areas of research. Because of this variety of research needs, using a supplier that offers a range of peptide services is important to fit your specific applications. Application areas such as antibody therapeutics, epitope mapping and enzyme profiling utilize peptides for antibody production, screening and assay development. Peptides modified with phosphorylation, acetylation or methylation enable monitoring of cell signaling events. Selection of optimal peptides for antibody or targeted assay development is accomplished through screening of crude peptide libraries. Optimal peptide sequences are then utilized to generate heavy peptide standards for relative and absolute targeted quantitation by mass spectrometry.

Highlights

• Low toxicity - peptides with acetate as the counter ion are less toxic in certain applications when compared with those with trifluoroacetate (TFA)1 .
• Application-specific - the 6-20-amino acid peptide length distribution fits most immunological applications.
• Convenient - peptides are provided in individual 2-D barcoded tubes in 96-tube plates, and each order includes a CD with the peptide sequences and positions in each plate.
• Flexible - optional services, including phosphorylation and acetylation, are available for studying signaling and regulatory proteins..

Applications:

• Epitope mapping of B- and T-cells
• Vaccine development
• Screening of peptide vaccine
• Biomarker discovery
• Cell signalling (kinase/protease studies)

Additional modifications upon request.