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Oligonukleotid szintézis, szintetikus biológia

IGÉNY SZERINTI OLIGONUKLEOTID SZINTÉZIS

Desalted custom synthesized DNA oligos are shipped dried down or hydrated according to your specifications. Synthesis scales up to 1 µmol are shipped the next business day. 5 µmol and 10 µmol scales are shipped within 5 business days.

 

Every oligo is deprotected and desalted to remove small molecule impurities. Oligos are quantified twice by UV spectrophotometry to provide an accurate measure of yield. Standard oligos are assessed by mass spectrometry for quality control (QC).

DNA Synthesis

Desalted custom synthesized DNA oligos are shipped lyophilized or customized according to your specifications. Synthesis scales up to 1 µmole are shipped the next business day. 5 µmole and 10 µmole scales are shipped within 5 business days.

 

Base Pricing
  • No setup fee for standard DNA synthesis
  • Large-scale synthesis (up to 10 grams) available.
ProductLength
25 nmole DNA Oligo 15 - 60 Bases
100 nmole DNA Oligo 10 - 90 Bases
250 nmole DNA oligo 5 - 100 Bases
1 umole DNA Oligo 5 - 100 Bases
5 umole DNA Oligo 5 - 100 Bases
10 umole DNA Oligo 5 - 100 Bases
 
Yield Guarantees for Standard Desalted DNA Oligos

At no extra charge, every oligo is deprotected and desalted to remove small molecule impurities. Oligos are quantitated twice by UV spectrophotometry to provide an accurate measure of yield. Mass yield, as measured in Optical density (OD), varies with length and decreases for shorter oligos. All oligos are quality control (QC) checked by mass spectrometry. The following yield guarantees are valid for oligonucleotides ≥20 bases. For more information on yields, please see the Technical Report, Oligonucleotide Yield, Resuspension, and Storage

 

Synthesis ScaleOligo LengthYield Guarantee
25 nmole DNA Oligo 15 - 60 Bases 3ODs
100 nmole DNA Oligo 10 - 90 Bases 6 ODs
250 nmole DNA oligo 5 - 100 Bases 15 ODs
1 umole DNA Oligo 5 - 100 Bases 50 ODs
5 umole DNA Oligo 5 - 100 Bases 250 ODs
10 umole DNA Oligo 5 - 100 Bases 500 ODs

 

Formulation Services and RxnReady (Premixed) Oligos

 
LabReady Oligo Service

IDT will rehydrate your oligos at a fixed concentration prior to shipment as part of our LabReady Oligo Service. For a fee, your 25, 100, or 250 nmole scale oligos are shipped hydrated at 100 µM in IDTE (1X TE Buffer), pH 8.0.

 

Custom Normalized Tubes

For ultimate convenience, specify the concentration at which you would like to receive your oligos. Buffer choices are RNase-free water*, or IDTE (1X TE Buffer), pH 8.0 or 7.5. Applies to 25 nmole, 100 nmole, 250 nmole, and 1 µmole scales. For resuspension of larger scales, please contact customer service at .

 

RxnReady Oligos

For a small mixing fee, have 2–6 standard desalt oligos premixed in a single tube according to your specifications. Oligos may contain modifications. Yields range between 5 and 50 nmole, and oligos can be shipped dried down or hydrated (in RNase-free water* or IDTE 8.0).
* To ensure longer stability of oligos, IDT recommends resuspension in TE.

 

Mixed Bases

IDT offers oligonucleotides containing randomized, or “mixed bases”. Mixed Bases are used in primers to bind to templates that contain variability or a mixture of sequences at the primer binding site. Mixed Bases can also be used to create diversity in clone libraries and in site-directed mutagenesis.
IDT offers two types of randomization, Machine Mixed and Hand Mixed Bases. Machine Mixed Bases can be made at any/all base sites at no additional charge. At these base positions, the synthesizer pulls an equal ratio of the desired bases, but their different coupling rates do not guarantee an equal ratio of incorporation.
Hand Mixed Bases provide customer-defined ratios of the desired bases, where coupling efficiencies are taken into account. For each mixture, IDT can incorporate up to 100 residues on the 100 nmole and 250 nmole scales and up to 40 residues on the 1 µmole scale, with a maximum of 4 unique Hand Mixes per oligonucleotide.When entering your sequence, use the “Mixed Base” tab at the bottom of the page. It lists the IUPAC-IUB symbols and is where custom mix ratios should be entered.

 

Other Custom Preparative Services

Annealing Services

Have complementary oligos annealed prior to shipment. Annealed oligos are supplied dry.
100nmole, 250nmole and 1µmole.

Custom Labels

Identify, sort, and distribute your tubes more quickly using your own set of customized labels. Using the drag-and-drop label designer, your custom label can include:

 

  • Sales Information (Purchase Order #, Name, University)
  • Manufacturing Data (Date, Product Name, Sequence)
  • Product Specifications (OD, # Bases, Molecular Weight, TM)
  • Lab Specific Info (Custom notes from your lab)
  • Image or Logo (upload your own, or use one of our stock images)

 

When you place your oligo order, the custom label option is also available at the bottom of the page.

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