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Thermo Scientific

NanoDrop 8000 UV-Vis Spectrophotometer

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Thermo Scientific NanoDrop 8000 UV-Vis Spectrophotometers are used in academic, biopharmaceutical, core and bioproduction facilities, to measure between 1 and 8 microvolume nucleic acid and protein samples simultaneously. The patented sample retention system allows you to assess the concentration and purity of samples as small as 1µL, while the multi sample capability allows you to analyze samples more efficiently.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

NanoDrop 8000 Spectrophotometer features:

  • Wide spectral range (220 - 750nm) for measuring a variety of samples types:
  • DNA and RNA (260nm)
  • Purified protein (280nm)
  • Toxicology assays and industrial dyes (490nm)
  • Gold nanoparticles (520nm)
  • Colorimetric protein assay (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
  • Optical Density measurements (600 nm)
  • No dilutions required
  • Only requires 1-2 µL of your sample
  • Calculates sample purity ratios (260/280 nm and 260/230 nm)
  • Measure up to 96 samples in less than six minutes
  • Choose to measure form 1 to 8 samples in one measurement cycle
  • Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
  • Patented sample retention system automatically optimizes pathlength to accommodate low and high concentrations (2.5 to 3,700 ng/µL dsDNA)
  • User-friendly software that includes custom methods and data export capabilities

 

Position Illuminator


 

When measuring samples in a 96-well format on the Thermo ScientificNanoDrop 8000 Spectrophotometer, an eight-channel pipettor is used to draw 1 ul samples from a microtiter plate which are then dispensed  onto the eight optical measurement surfaces. 

The Sample Position Illuminator keeps track of the samples to be measured by lighting up wells from beneath the plate. This helps the user readily identify which wells to draw from for the next measurement, thus reducing fatigue and human error.

The sample position illuminator is simple to use. Prior to measurement, the user selects the samples to be measured from the on screen position indicator (selected wells are in green).

 

The sample position illuminator then lights up the corresponding wells of the plate that are to be measured:

The user then simply draws from the illuminated wells.

 

Additional Features:

  • Auto advance: This increases measurement throughput by automatically
  • lighting up the next column to be measured.
  • "Cherry picking": User can select any single or multiple wells which are
  • then lit up by the sample position illuminator.
  • Measurement limits: The user can define upper and lower concentration
  • (or absorbance) limits such that a well will begin to flash if the 
  • measurement falls out of adefined range (e.g. 100ng/ul ±20ng/ul).

Performance

Data from studies using the Thermo Scientific NanoDrop 8000 Spectrophotometer are below:

Műszaki specifikációk

 

Sample size 1 µl
Sample Number up to 8
Path lengths 1 mm and 0.2 mm
Light Source Xenon flash lamp
Detector Type 2048-element linear silicon CCD
Wavelength range 220-750 nm
Wavelength accuracy 1 nm
Wavelength resolution 3 nm (FWHM at Hg 546 nm)
Recommended Sample Volume, Nucleic acid 1 µl
Recommended Sample Volume, Proteins 2 µl
Absorbance  Precision 0.003 absorbance (1 mm path)
Absorbance Accuracy 3% (at .74 absorbance at 350 nm)
Absorbance Range 0.05-75 (10 mm equivalent absorbance)
Detection Limit 2.5 ng/µl (dsDNA)
Maximum Concentration 3700 ng/µl (dsDNA)
Measurement Cycle Time 20 seconds (for 8 samples)
Dimensions (footprint) 24 cm X 32 cm
Weight 3.4 kg
Sample Pedestal Material of Construction 303 stainless steel and quartz fiber
Operating voltage 12 vdc
Operating Power Consumption 30 W
Standby Power Consumption 6 W
Software Compatibility Professional versions of Windows 7 (32 bit and 64 bit), Windows Vista (32 bit), 
Windows XP (32 bit)
UL/CSA and CE approval All units
Recommended sample volume, Nucleic acid 1ul
Recommended sample volume, Proteins 2ul
Applikációk
  • The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
  • Measuring Viability Decline in Animal Cell Suspension Cultures Using the Thermo Scientific NanoDrop 2000 Spectrophotometer.
  • Nanoparticle Characterization Resources for the NanoDrop 2000/c Spectrophotometer
  • Quantification of Gold Nanoparticles
  • Differences in Bacterial Optical Density Measurements between Spectrophotometers
  • Activity Measurements of Pentose Phosphate Pathway Enzymes Using Kinetics
  • Quality Control during Library Construction for de novo Next-Gen Sequencing Platforms
  • Generation of a Standard Curve Based Custom Method using the NanoDrop 2000/2000c: Lowry Assay Case Study
  • Quality Control of Industrial Dye Products Using Absorbance Spectroscopy
  • Detection and Avoidance of Polysaccharides in Plant Nucleic Acid Extractions
  • Quality Control of Small Molecule Pharmaceuticals using Spectrophotometry
  • Pierce 660 nm Micro-Volume Assay Advantages
  • Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies
  • Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence (Poster)
  • Microvolume Quantification of Nucleic Acids in Molecular Diagnostics (Poster)
  • Small Molecule Crystallography
  • Microgenomic Expression Profiling
  • Quantitative Real Time RT PCR
  • Nucleic Acid and Fluorescent Dye Concentration Measurement
  • HLA Typing: Template Quality Control for SSP and SSO
Videók, ismertetők

Brochure

 

How It Works

See how you can measure more samples in less time

 

PUBLICATIONS

 

The Latest in Laboratory Apps

Lab Manager Magazine, October 2013


A Novel Process for Developing Fully Human Monoclonal Antibodies

PharmaTech, November 2012


Comparing Microvolume and Cuvette Based Measurements of Microbial Cell Cultures

Lab Manager Magazine, October 2011


Assuring Success with qPCR

Bioscience Technology, September 2011


Assuring Reliability of qPCR & RT-PCR Results

Genetic Engineering & Biotechnology News, September 2011


Empowering PCR

Bioscience Technology, September 2011


Global Incentives Seek to Buoy Innovation

Genetic Engineering & Biotechnology News, September 2011


Molecular Diagnostics: Critical Issues for Successful Translation from the Bench to the Clinic

Pharma Bio World, August 2011


PCR Gets Personal, Faster

Pharmaceutical Formation & Quality, January 2011


qPCR y RT-qPCR con garantia de exito

FARMESPANA INDUSTRIAL, January 2011


Use of Microvolume Analysis in Processing

Genetic Engineering News, March 2010


Current Protocols in Molecular Biology: "Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy"

Presented here are the first two pages of this Protocol. Please see Wiley InterScience for full protocol details.


Current Protocols in Protein Science: "Microvolume Spectrophotometric and Fluorometric Determination of Protein Concentration"

Presented here are the first two pages of this Protocol. Please see Wiley InterScience for full protocol details.


The power to choose expands possibilities for measurement

eLab, June 2009


"Small, easy and fast": when 'less is more' in Spectrophotometry

BioTech International, March 2009


Microsample quantitation technology for molecular diagnostics

Clinical Laboratory International, October 2008


Tissue typing - just a drop in the ocean

MedLab News, October 2008


Real-Time PCR Bridges Molecular Biology and Clinical Diagnostics

Clinical Lab Products, October 2008


Sample Quantitation with Microvolume Tools

Genetic Engineering News, September 2008


Its a question of conservation

Laboratory News, March 2008


Spectrophotometry

Biocompare, March 2008


NanoDrop makes low volume analysis a cinch

LabTechnologist.com, March 2008


NanoDrop 1000 UV-Vis Spectrophotometer From NanoDrop Technologies

BioCompare, September 2007


The State of Microsample Quantitation

Genetic Engineering News, April 2007


Life Science Technologies, Nanobiotechnology: An Incredible Voyage for the Life Sciences

Science, November 2006


Photonics Takes on the Challenge of Microanalysis

BioPhotonics International, January 2006


Microgenomics- Into the Mainstream

BioScience Technology, July 2005


Expression Profiling from a Limited Cell Mass

Genetic Engineering News, March 2005


Advances in Microgenomics

Genetic Engineering News, February 2005


Just Measure It: Cuvetteless Spectrophotometry of 1ul Samples

Nature, March 2005


1ul Micro-Spectrophotometry: A Versatile and Enabling Technology

Nature, January 2005


Spectrophotometry's Next-Generation Technology

American Biotechnology Laboratory, December 2003

 

CITATIONS

 

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