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ProteinSimple

Wes

ps_wes
4857592-2-400x300
Simple Westerns just got even simpler!
A capillary MW based protein separation with chemiluminescent detection and outstanding throughput - up to 25 samples per run in less than 3 hours!

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Add your samples and primary antibody or labeling reagent to the pre-filled assay plate. Pop in Wes'™ capillary cartridge and load the plate. Push start and in just 3 hours get quantitated size-based separation data on up to 25 samples. After the run, toss the used cartridge and plate and you're done.

Műszaki specifikációk

WHICH SYSTEM IS RIGHT FOR YOU?

Simple Western SystemWesSally SuePeggy SueNanoPro 1000
Simple Western size assays        
Simple Western charge assays        
Maximum samples per run 25 96 96 96
Run time for max samples (size) < 3 hours 14-19 hours 11-19 hours N/A
Run time for max samples (charge) N/A N/A 11 hours 11 hours
Sample cooling (size) N/A 10°C 10°C N/A
Sample cooling (charge) N/A N/A 3°C 3°C
Operating humidity range 20-60% relative, non-condensing
Operating temp range 18-24°C
Power 100-230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 84 x 94 x 61 cm 84 x 94 x 61 cm 84 x 94 x 61 cm
Part number 004-600 004-700 004-800 004-109
 
 Total ProteinImmunoassay
Description Size Specification Size Specification Charge Specification
Sample required 0.3-1.2 µg lysate 0.6-1.2 µg 0.6-1.2 µg
Size or pI range 12-230 and 66-440 kDa 12-230 and 66-440 kDa Widest gradient ranges
from pI 3 to pI 10
Sizing CV <10% <10% <10%
Intra-assay CV <15% <15% <20%
Inter-assay CV <20% <20% <20%
Resolution ± 10% difference in
molecular weight
± 10% difference in
molecular weight
± 0.1 pI units
Quantitation CV <20% <20% <20%
Dynamic range 2-3 logs 3 logs 3 logs
Sensitivity ng Low pg Low pg
Capillary 5 cm, 100 µm, 400 nL
 

 

 

Applikációk

TECHNOLOGY & APPLICATIONS

SIMPLE WESTERN ASSAYS ON WES

FIGURE 1.  Total Protein detection (left) and Immune detection (right) of decreasing concentrations of DNAK in Hela lysate and negative controls (15, 7.5 and 3.75 µg/mL in the total protein assay; 0.015, 0.0075 and 0.00375 µg/mL in the immunoassay).

FIGURE 2.  GST-labeled AKT was used to generate a standard curve for quantitation of endogenous AKT. AKT-GST was spiked into the Jurkat lysate at decreasing concentrations (250—0 pg/µL) and both the labeled and endogenous proteins were detected using an AKT1 monoclonal antibody. Linear regression analysis was performed and the endogenous concentration of AKT in the samples was calculated as 21 pg/µL.

FIGURE 3.  Simple Western 90 kDa System Control and endogenous C-Abl, BCR-Abl, and CBP in K562 cells detected simultaneously by Wes.

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