How Lumit™ Immunoassays Work
Lumit™ Immunoassays are based on NanoLuc® Binary Technology (NanoBiT®). In Lumit™ Immunoassays, antibodies are chemically labeled with the small and large subunits of NanoLuc® Luciferase, known as SmBiT and LgBiT, respectively. In the presence of an analyte, the two antibodies come into close proximity, allowing SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal.
Lumit™ Immunoassay Products
No-Wash Detection of Spike (RBD) and Human ACE2 Interaction
- Detect neutralizing antibodies, peptides, or small molecule inhibitors
- Linear response in the picomolar to ~2nM range of SARS-CoV-2 RBD and hACE2 proteins
- No wash, transfer or immobilization steps.
- Available as early access material; please contact us for more information.
No-Wash Immunoassay for IL-1β Detection in Cells
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a standard plate-reading luminometer
- Scalable to 96- or 384-well plates
Lumit™ Immunoassays for cytokine detection quantitatively measure target analytes in cell culture samples with a simple, no-wash assay protocol. The assays can be performed directly on cell culture samples or on medium transferred from cell plates.
Insulin and glucagon are key target analytes measured in metabolism research. Secreted from β and α cells of pancreatic islets, respectively, the two hormones are vital for regulating glucose levels in the body. The Lumit™ Immunoassays for metabolic regulators quantitatively measure insulin and glucagon in cell culture samples with a scalable, no-wash assay protocol. These assays are ideally suited to process samples from glucose-stimulated insulin secretion and glucagon secretion experiments, including high-throughput perifusion assays.
Measure Neonatal Fc Receptor Binding
- No wash or transfer: Simple add-mix-read protocol
- Results in less than one hour
- Scalable and high throughput: Perform assays in 96-well or 384-well plates
- Add-and-mix assay format—no washing steps, no immobilization to plates, beads or other surfaces, 30–90 minutes total time
- Simple luminescence-based detection
- Solution-based, sensitive assays with wide dynamic range
No-Wash, One-Plate Immunoassay for Kinase Pathway Analysis
- Set 1: Lumit™ Anti-Mouse Ab-LgBiT/Lumit™ Anti-Rabbit Ab-SmBiT
- Set 2: Lumit™ Anti-Mouse Ab-SmBiT/Lumit™ Anti-Rabbit Ab-LgBiT
- Starter Kit: Includes all four Set 1 & Set 2 Lumit™ Secondary Antibodies
- Primary antibodies supplied by the user
Separate Components for the Lumit™ Immunoassay Cellular Systems
- These components are a part of the Lumit™ Immunoassay Cellular Systems, which bundle the Lumit™ Immunoassay Lysis and Detection Kit reagents with specific Lumit™ Secondary Antibody pairs
- Cannot be used with primary antibodies from the same species
- Sensitive, solution-based immunoassays—no washing steps, no immobilization to plates, beads or other surfaces