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Promega

Lumit™ Immunoassays for Research

vb2500_lumit--immunoassay-labeling-kit_3
Képernyőkép 2021-03-17 155745
Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods including sandwich ELISAs and Western blots. Lumit™ assays are sensitive, have broad dynamic range and can be completed in as little as 30 minutes, making them a compelling alternative to labor-intensive ELISA and Western blot methods.

Advantages of Lumit™ Assays over conventional immunoassays include:

Simple add-mix-read protocol with no washing steps
Direct analyte measurement in the cell culture plate or on medium removed from the cells
No immobilization to plates, beads or other surfaces required
Sensitive luminescence detection with wide dynamic range
Lumit™ Assays are available as catalog items, early-access materials or by custom order. Early-access materials include labeling and detection reagents that allow you to label antibodies and proteins with SmBiT and LgBiT and develop your own immunoassays, and pre-built immunoassays for detection of specific targets. See the product pages below for specifics on each assay type

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

How Lumit™ Immunoassays Work

Lumit™ Immunoassays are based on NanoLuc® Binary Technology (NanoBiT®). In Lumit™ Immunoassays, antibodies are chemically labeled with the small and large subunits of NanoLuc® Luciferase, known as SmBiT and LgBiT, respectively. In the presence of an analyte, the two antibodies come into close proximity, allowing SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal.

 

Lumit™ Immunoassay Products

Lumit™ SARS-CoV-2 Spike RBD: hACE2 Immunoassay

No-Wash Detection of Spike (RBD) and Human ACE2 Interaction

  • Detect neutralizing antibodies, peptides, or small molecule inhibitors
  • Linear response in the picomolar to ~2nM range of SARS-CoV-2 RBD and hACE2 proteins
  • No wash, transfer or immobilization steps.
  • Available as early access material; please contact us for more information.

Lumit™ IL-1β Human/Mouse Immunoassay

No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates

 

Cytokine Detection with Lumit™ Immunoassays

Lumit™ Immunoassays for cytokine detection quantitatively measure target analytes in cell culture samples with a simple, no-wash assay protocol. The assays can be performed directly on cell culture samples or on medium transferred from cell plates.

 

Detection of Metabolic Regulators with Lumit™ Immunoassays

Insulin and glucagon are key target analytes measured in metabolism research. Secreted from β and α cells of pancreatic islets, respectively, the two hormones are vital for regulating glucose levels in the body. The Lumit™ Immunoassays for metabolic regulators quantitatively measure insulin and glucagon in cell culture samples with a scalable, no-wash assay protocol. These assays are ideally suited to process samples from glucose-stimulated insulin secretion and glucagon secretion experiments, including high-throughput perifusion assays.

 

Lumit™ FcRn Binding Immunoassay

Measure Neonatal Fc Receptor Binding

  • No wash or transfer: Simple add-mix-read protocol
  • Results in less than one hour
  • Scalable and high throughput: Perform assays in 96-well or 384-well plates

 

Lumit™ Immunoassay Labeling Kit and Detection Reagents

  • Add-and-mix assay format—no washing steps, no immobilization to plates, beads or other surfaces, 30–90 minutes total time
  • Simple luminescence-based detection
  • Solution-based, sensitive assays with wide dynamic range

 

Lumit™ Immunoassay Cellular Systems

No-Wash, One-Plate Immunoassay for Kinase Pathway Analysis

  • Set 1: Lumit™ Anti-Mouse Ab-LgBiT/Lumit™ Anti-Rabbit Ab-SmBiT
  • Set 2: Lumit™ Anti-Mouse Ab-SmBiT/Lumit™ Anti-Rabbit Ab-LgBiT
  • Starter Kit: Includes all four Set 1 & Set 2 Lumit™ Secondary Antibodies
  • Primary antibodies supplied by the user

Lumit™ Immunoassay Lysis and Detection Kits and Secondary Antibodies

Separate Components for the Lumit™ Immunoassay Cellular Systems

  • These components are a part of the Lumit™ Immunoassay Cellular Systems, which bundle the Lumit™ Immunoassay Lysis and Detection Kit reagents with specific Lumit™ Secondary Antibody pairs
  • Cannot be used with primary antibodies from the same species
  • Sensitive, solution-based immunoassays—no washing steps, no immobilization to plates, beads or other surfaces

 

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