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Promega

Sejtéletképesség és metabolizmus

Our wide selection of bioluminescent enzyme substrates for proteases and metabolic enzymes such as CYPs and MAO, among others, provide powerful tools for the ADME-Tox screen.
Get more data in less time when you multiplex cell health biomarkers. Measure one or multiple biomarkers in vitro or in situ to detect early or late events of apoptosis and get a quick status update on targeted cells.
Cell viability assay kits for easy measurement of viability in cell culture, 3D microtissues, bacterial cultures and virus-infected cells. Simple "add-mix-measure" protocols and convenient luminescent readout allow you to easily compare cell viability data from well-to-well, from plate-to-plate and from day-to-day.
Promega offers multiple readouts for quantifying cytotoxicity, including luminescent and fluorescent assays that can be multiplexed with apoptosis or viability assays to help determine mechanism of cell death.
NAD+, NADP+, NADH and NADPH are important cofactors for many enzymes involved in key cellular pathways, and quantitation of these dinucleotides is a standard approach to monitor enzyme activity directly or indirectly. Promega offers a number of systems to detect and quantify these important molecules.
Promega offers assays for detecting glutathione, measuring the ratio of reduced to oxidized glutathione and detecting changes in ROS as an indicator of cell health or cell signaling events.
Reporter vectors and cell lines containing response elements for monitoring activity of key cell signaling pathways.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

ADME Assays 

P450-Glo™ Assays

Luminescent assays for measuring cytochrome P450 activity, including systems with suitable for cell-based assays and DMSO-tolerant substrates.

UGT Activity Assays

The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase activity. Complete assay systems are available for UGT1A1 and UGT2B7.

Pgp-Glo™ Assay

A luminescence-based assay that detects the effects of compounds on recombinant human Pgp in a cell membrane fraction.

MAO-Glo™ Assay Systems

A homogeneous luminescent method for measuring MAO activity from recombinant and native sources, as well as the effects of test compounds on MAO activities. 

Apoptosis Assays 

CellTox™ Green Cytotoxicity Assay

A single-reagent fluorescent assay with a flexible protocol to perform kinetic or endpoint cytotoxicity analysis after extended exposure.

Determine Mechanism of Cell Death
The ApoTox-Glo™ Assay  measures 3 key independent biomarkers for viability, cytotoxicity and apoptosis in the same sample well.
Fluorescent Caspase Assay

The Apo-ONE® Homogeneous Caspase-3/7 Assay is a fast, sensitive, fluorescent apoptosis detection system featuring a homogeneous format.

Luminescent Caspase Assays

The Caspase-Glo® Assays monitor toxic effects of compounds at high-throughput capacities, or one sample at a time, in as little as 15 minutes. 

Cell Viability Assays 

RealTime-Glo™ MT Cell Viability Assay

Monitor cell viability out to 72 hours in the same well. No cell washing, media removal or further additions required. Add at plating, during treatment or at the end of treatment. 

CellTiter-Glo® 3D: Cell Viability for 3D Cultures

Accurate cytotoxicity determination validated for 3D microtissue cultures. Easy assay implementation. Simple, 30-minute protocol.

CellTiter-Glo® Assay

Directly measure the key energy metabolite ATP, resulting in sensitive quantitation of the number of healthy cells in culture using this simple assay.

MultiTox Multiplex Assays

Control variability by quantifying the number of live and dead cells within a single well. 

Cytotoxicity Assays 

CellTox™ Green Cytotoxicity Assay

A single-reagent fluorescent assay with a flexible protocol to perform kinetic or endpoint cytotoxicity analysis after extended exposure.

Determine Mechanism of Cell Death
The ApoTox-Glo™ Assay 

measures 3 key independent biomarkers for viability, cytotoxicity and apoptosis in the same sample well.

MultiTox Multiplex Assays

Control variability by quantifying the number of live and dead cells within a single well.

CytoTox-Fluor™ Cytotoxicity Assay

A single-reagent-addition, homogeneous, fluorescent assay that measures dead-cell protease activity released from cells that have lost membrane integrity.

CytoTox-ONE™ Homogeneous Membrane Integrity Assay

A rapid, fluorescent measure of the release of LDH from cells with damaged membranes. Can be used to measure released LDH in plate wells containing a mix of viable and damaged cells.

CytoTox-Glo™ Cytotoxicity Assay

A luminescent cytotoxicity assay that measures the relative number of dead cells in cell populations. 

Metabolism Assays 

NAD(P)H-Glo™ Detection System

A homogeneous bioluminescent assay that quantifies NADH and NADPH and does not discriminate between them. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.

NAD/NADH-Glo™ Assay

A homogeneous, bioluminescent assay for directly detecting total oxidized and reduced nicotinamide adenine dinucleotides (NAD+and NADH, respectively) and determining their ratio in biological samples.

NADP/NADPH-Glo™ Assay

A homogeneous, bioluminescent assay for directly detecting total oxidized and reduced nicotinamide adenine dinucleotide phosphates (NADP+ and NADPH, respectively) and determining their ratio in biological samples.

 

Inflammation Assay

Caspase-Glo™ 1 Inflammasome Assay

A homogenous, multiwell plate-based assay to selectively detect caspase-1 activity as a biomarker for inflammasome activation. This assay is a faster, simpler alternative to Western blot and ELISA assays for detecting cleaved caspase-1 and interleukin 1β 

Mitochondrial Function Assays

A multiplexed, cell-based assay to monitor mitochondrial health. Measures ATP and a membrane integrity biomarker to distinguish mito-toxic mechanisms from other cytotoxic events.

Mitochondrial ToxGlo™ Assay

A cell-based multiplexed method that measures ATP as an indicator of mitochondrial function and uses a membrane integrity biomarker to distinguish primary mitochondrial dysfunction from secondary cytotoxic events. 

Oxidative Stress Assays 

ROS-Glo™ H2O2 Assay

A rapid, sensitive bioluminescent assay to identify conditions or test compounds that alter ROS levels. Eliminates false-hits associated with HRP methods.

GSH-Glo™ Glutathione Assay

Detects and quantifies glutathione in cells or in a variety of biological samples, providing a sensitive alternative to colorimetric and fluorescent methods. Suitable for HTS.

GSH/GSSG-Glo™ Assay

Detects and quantitates total glutathione and GSSG to allow calculation of ratio of reduced (GSH) to oxidized glutathione (GSSG). 

Toxicity Pathway Analysis 

GloResponse™ Luciferase Reporter Cell Lines

State-of-the-art luciferase reporter technology integrated into a cell line allows rapid development of reporter assays based on the pathway of interest regulating the luciferase gene. Cell lines containing CRE, NFAT, NF-κB and GAL4UAS response elements are available.

Reporter Vectors for Toxicity Pathway Analysis

These vectors report the activity of a variety of signaling pathways using the luc2 firefly luciferase gene. Each vector contains a response elements upstream of a minimal promoter (minP). Choose the appropriate vector for your signaling pathway of interest.

Custom Materials for Toxicity Pathway Analysis

We offer a number of specific Toxicity Pathway Reporters and Cell Lines, which are available for sale by quote.

 

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