Fast and flexible
Cell3™ Xtract has a simple and flexible protocol that requires no specialist equipment and with just 90 minutes of processing time will speed up your extractions.
Whether you are working with 1ml or 10ml you can extract all cfDNA in one extraction. With a small elution volume of just 35ul you can avoid the need for a DNA concentration step.
Isolate cfDNA from a wide range of samples types: plasma, cerebrospinal fluid (CSF), saliva and amniotic fluid.
Efficient cfDNA extraction without carrier RNA ensures accurate quantification of your extracted cfDNA for sensitive downstream applications like NGS.
The utility of cell-free DNA (cfDNA) in both translational research and diagnostic settings has increased dramatically since its discovery. CfDNA is typically found in short fragment sizes of ~160 bp and low quantities of 1 to 100 ng/ml in plasma. It is therefore critical that sufficient cfDNA of good quality can be extracted for downstream applications like NGS and qPCR.
To address this need Nonacus has developed a fast, accurate and flexible cfDNA extraction protocol designed to optimise cfDNA extraction from plasma and maximise yield.
Cell3™ Xtract has a simple and flexible workflow which allows 1-10 ml of biological sample to be processed within 90 minutes.
With a small elution volume of just 35ul, regardless of your starting volume, you can avoid the need for a DNA vacuum concentration step. This means you can add more cfDNA to your downstream applications including ddPCR and NGS enabling more sensitive detection of variants.
Accurate quantification of extracted cfDNA is important for downstream applications, particularly Next Generation Sequencing (NGS) where input amounts for assays can be critical. Many companies use carrier RNA to improve cfDNA extraction which can lead to an overestimate of cfDNA quantification and compromise the effectiveness of NGS library preparation.
The Cell3™ Xtract kit does not require carrier RNA to optimise cfDNA isolation enabling accurate quantification of cfDNA and efficient library preparation.
We compared Cell3™Xtract to spin column and bead-based cfDNA extraction kits from four other companies (companies Q, A, O, M).
Using cfDNA extracted from 1ml plasma from a pregnant woman carrying a male fetus, qPCR, DNA concentration and fragment size analysis showed that, in the majority of cases, Cell3™ Xtract outperformed other manufacturers.
Figure 1. Column-based extraction kits: qPCR data revealed that the Cell3™ Xtract kit performed on par with the Company Q kit and outperformed the Company A kit. However, the use of carrier RNA in the Company Q kit resulted in a 50-fold higher DNA concentration measurement and the appearance of a high molecular weight peak in the fragment analysis electropherogram.
Figure 2. Bead-based extraction kits: qPCR and DNA concentration data showed that the Cell3™ Xtract kit performed better than the Company M kit and on par with the Company O kit.
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