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Cell3™ Xtract cfDNA isolation kit

Efficient cell-free DNA (cfDNA) extraction
The Cell3™ Xtract kit enables fast and flexible extraction of cell free DNA (cfDNA) from a range of biological samples ensuring high quality cfDNA & ctDNA for research and clinical applications.


Fast and flexible


Cell3™ Xtract has a simple and flexible protocol that requires no specialist equipment and with just 90 minutes of processing time will speed up your extractions.

Maximise sample input


Whether you are working with 1ml or 10ml you can extract all cfDNA in one extraction. With a small elution volume of just 35ul you can avoid the need for a DNA concentration step.

Supports multiple uses


Isolate cfDNA from a wide range of samples types: plasma, cerebrospinal fluid (CSF), saliva and amniotic fluid.

Accurate quantification


Efficient cfDNA extraction without carrier RNA ensures accurate quantification of your extracted cfDNA for sensitive downstream applications like NGS.

Optimsing cell-free DNA extraction from plasma

The utility of cell-free DNA (cfDNA) in both translational research and diagnostic settings has increased dramatically since its discovery. CfDNA is typically found in short fragment sizes of ~160 bp and low quantities of 1 to 100 ng/ml in plasma. It is therefore critical that sufficient cfDNA of good quality can be extracted for downstream applications like NGS and qPCR.

To address this need Nonacus has developed a fast, accurate and flexible cfDNA extraction protocol designed to optimise cfDNA extraction from plasma and maximise yield.

Large volume cell-free DNA extraction

Cell3™ Xtract has a simple and flexible workflow which allows 1-10 ml of biological sample to be processed within 90 minutes.

The flexible input volume allows for increased recovery and concentration of cfDNA from an entire sample, avoiding the necessity for multiple 1 ml extractions. No specialist equipment such as magnets or vacuum manifolds are required.

Obtain higher concentration cfDNA

With a small elution volume of just 35ul, regardless of your starting volume, you can avoid the need for a DNA vacuum concentration step. This means you can add more cfDNA to your downstream applications including ddPCR and NGS enabling more sensitive detection of variants.

Cell3 Xtract Kit
Cell3 xtract

Accurate quantification of cfDNA

Accurate quantification of extracted cfDNA is important for downstream applications, particularly Next Generation Sequencing (NGS) where input amounts for assays can be critical. Many companies use carrier RNA to improve cfDNA extraction which can lead to an overestimate of cfDNA quantification and compromise the effectiveness of NGS library preparation.

The Cell3™ Xtract kit does not require carrier RNA to optimise cfDNA isolation enabling accurate quantification of cfDNA and efficient library preparation.

Superior technical performance

We compared Cell3™Xtract to spin column and bead-based cfDNA extraction kits from four other companies (companies Q, A, O, M).

Using cfDNA extracted from 1ml plasma from a pregnant woman carrying a male fetus, qPCR, DNA concentration and fragment size analysis showed that, in the majority of cases, Cell3™ Xtract outperformed other manufacturers.


Figure 1. Column-based extraction kits: qPCR data revealed that the Cell3™ Xtract kit performed on par with the Company Q kit and outperformed the Company A kit. However, the use of carrier RNA in the Company Q kit resulted in a 50-fold higher DNA concentration measurement and the appearance of a high molecular weight peak in the fragment analysis electropherogram.


Figure 2. Bead-based extraction kits: qPCR and DNA concentration data showed that the Cell3™ Xtract kit performed better than the Company M kit and on par with the Company O kit.


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