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Thermo Scientific

NanoDrop One/OneC UV-Vis Spectrophotometer

Quantify and qualify DNA, RNA, and protein samples with only 1-2 µL and have full-spectral data in seconds using the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis spectrophotometer.

Prevent costly delays — identify contaminants, obtain corrected concentrations, and enjoy success in your downstream applications
Collect data fast — Auto-Blank and Auto-Measure modes save time while organizing data
Save bench space — user-friendly local control with sleek, standalone design means no PC required


Prevent costly delays with an enhanced understanding of sample quality using the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometers. Innovative Thermo Scientific™ Acclaro™ Sample Intelligence technology is built into every instrument for improved measurement accuracy and contaminant identification. Quantify and qualify DNA, RNA, and protein samples  in seconds with only 1-2 µL, and obtain full-spectral data before you decide to use samples in downstream applications. Additional cuvette position also available.

Acclaro Sample Intelligence Technology
Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.


Acclaro technology allows you to:

Verify sample quality in seconds — identify sample contaminants and obtain corrected concentration results

Access information through alerts — receive instant feedback about sample quality with on-demand technical support and guided troubleshooting

Rely on accurate results — ensure measurement integrity with embedded sensor and digital image analysis

NanoDrop One/Onec Enhancements

Ergonomic, standalone design — integrated Android™ tablet eliminates the need for a separate computer and provides modern connectivity for seamless data transfer via Wireless*, Ethernet or USB.

Auto-Measure and Auto-Blank functions — for fast and easy measurements of multiple samples. Streamline workflows with instant measurements that occur when the pedestal arm is down. These functions can be turned ON or OFF with a single tap on the screen.

Wider dynamic range — no dilutions required for highly concentrated samples (dsDNA at 27,500 ug/µL) with auto-range pathlength technology.

Integrated Learning Center — an archive of answers with technical support documents and educational animations at your fingertips.


NanoDrop Onec Instrument Highlights
  • Contains both pedestal and cuvette positions to expand experimental flexibility and increase the dynamic range.
  • Measure dilute samples, perform kinetics experiments and take optical density measurements of bacterial cultures.
  • Cuvette position includes temperature control and stirring.
NanoDrop One/Onec Spectrophotometer capabilities:
  • Wide spectral range (190-850 nm) for measuring a variety of samples types:
    • Peptides (205 nm)
    • DNA and RNA (260 nm)
    • Purified protein (280 nm)
    • Toxicology assays and industrial dyes (490 nm)
    • Gold nanoparticles (520 nm)
    • Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
    • Optical Density measurements (600 nm)
  • Combining the patented** sample-retention system with cuvette capability accommodates low and high concentrations (2.0 - 27,500 ng/ µL dsDNA and 0.06 - 820 mg/mL BSA)
  • Pedestal measurements that require only 0.5 - 2 µL of your sample and do not require sample dilution, even for highly concentrated samples
  • Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
  • User-friendly software that includes custom methods and data export capabilities
  • Instrument comes with a 2-year warranty for worry-free support


Műszaki specifikációk

NanoDropOne Specs

Videók, ismertetők

NanoDrop One brochure


Comparison of discontinued NanoDrop1000, current NanoDrop 2000/c and newly-released NanoDrop One/c


Pipette. Measure. Know. Watch the video introducing Thermo Scientific NanoDrop One Microvolume UV-Vis spectrophotometers.




  • The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
  • Measuring Viability Decline in Animal Cell Suspension Cultures Using the Thermo Scientific NanoDrop 2000 Spectrophotometer.
  • Nanoparticle Characterization Resources for the NanoDrop 2000/c Spectrophotometer
  • Quantification of Gold Nanoparticles
  • Differences in Bacterial Optical Density Measurements between Spectrophotometers
  • Activity Measurements of Pentose Phosphate Pathway Enzymes Using Kinetics
  • Quality Control during Library Construction for de novo Next-Gen Sequencing Platforms
  • Generation of a Standard Curve Based Custom Method using the NanoDrop 2000/2000c: Lowry Assay Case Study
  • Quality Control of Industrial Dye Products Using Absorbance Spectroscopy
  • Detection and Avoidance of Polysaccharides in Plant Nucleic Acid Extractions
  • Quality Control of Small Molecule Pharmaceuticals using Spectrophotometry
  • Pierce 660 nm Micro-Volume Assay Advantages
  • Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies
  • Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence (Poster)
  • Microvolume Quantification of Nucleic Acids in Molecular Diagnostics (Poster)
  • Small Molecule Crystallography
  • Microgenomic Expression Profiling
  • Quantitative Real Time RT PCR
  • Nucleic Acid and Fluorescent Dye Concentration Measurement
  • HLA Typing: Template Quality Control for SSP and SSO

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