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Opentrons

Opentrons OT-2

OT2-1
OT2-2
The OT-2: Your Personal Pipetting Robot

Get started automating your lab today

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

 

 

Lab automation has never been easier.

The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.

 

Small Footprint, Big Impact

Fits on half a standard lab bench

Metric (width, depth, height)63 cm x 57 cm x 66 cm
Imperial (width, depth, height)25 in x 22.5 in x 26 in

Performs like robots costing 10x more

Accuracy15% at 1 μL
Precision5% at 1 μL
 

Choose your hardware and pipettes

11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes

Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available

Use your existing labware and reagents

Reagent and consumables agnostic

Compatible with any ANSI/SLAS-compliant labware

 

Choose the protocol method that’s right for you

A protocol is the code that runs the robot through your experiment. We’ve built different ways to create a protocol that are accessible for everyone – from the automation rookie to the experienced Python coder.

 

Space for two pipettes

Reliable liquid transfers from 1–1000 µL with our swappable single- and 8-channel pipettes

 

OT2 3

 

Műszaki specifikációk

 

OT2 4

Dimensions

63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench

OT2 5

Weight

48 kg or 105.8 lb

Pipette configurations

Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.

Pipette volumes and specs

Single-channel: 1-1000 µL
8-channel: 1-300 µL

 
 

11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.

Connectivity

WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0

Video

Integrated video camera (Included for future or custom functionality)

Audio

Integrated audio system (Included for future or custom functionality)

Power requirements

Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, 

Frame composition

Rigid steel and CNC aluminum design

Operating temperature & relative humidity

Recommended 20-24 °C
Recommended 40-60% RH

 
Time to fill 96-well plate
PipetteDistribute,
one tip
Transfer,
same tip
Transfer,
new tip
P20 8-Channel 0:22 1:35 4:19
P300 8-Channel 0:26 1:38 4:24
P20 Single-Channel 3:12 13:10 36:01
P300 Single-Channel 4:01 13:39 41:31
P1000 5:54 15:21 44:50
Single-channel accuracy & precision
PipetteVolume Random Error  Systematic Error 
    %D   µl %CV   µl
P20 1
10
20
±15%
±2%
±1.5%
  0.15µl
0.2µl
0.3µl
±5%
±1%
±0.8%
  0.05µl
0.1µl
0.16µl
P300 20
150
300
±4%
±1%
±0.6%
  0.8µl
1.5µl
1.8µl
±2.5%
±0.4%
±0.3%
  0.05µl
0.6µl
0.9µl
P1000 100
500
1000
±2%
±1%
±0.7%
  2.0µl
5.0µl
7.0µl
±1%
±0.2%
±0.15%
  1µl
1µl
1.5µl
8-channel accuracy & precision
PipetteVolume Random Error  Systematic Error 
    %D   µl %CV   µl
P20 1
10
20
±20%
±3%
±2.2%
  0.2µl
0.3µl
0.44µl
±10%
±2%
±1.5%
  0.1µl
0.2µl
0.3µl
P300 20
150
300
±10%
±2.5%
±1.5%
  2.0µl
3.75µl
4.5µl
±4%
±0.8%
±0.5%
  0.8µl
1.2µl
1.5µl
Sterility

Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights

Viscosity
For best practices on automating viscous liquid handling, review our application note
 
API compatibility

Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.

Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.
Tip compatibility

Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility

Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.

One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use

60 day, 100% risk free
Certifications

Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)

Example Commands

Tip Handling

  • Pick up tip
  • Drop tip
  • Return tip
  • New tip (always, never, once)
  • More…

Liquid Control
  • Aspirate
  • Dispense
  • Aspirate speed
  • Dispense speed
  • Blowout
  • Touch tip
  • Mix
  • Air gap

Utility

  • Move to
  • Delay
  • User-specified pause
  • Homing
  • Comment


Transfer

  • Basic transfer
  • Volumes > Pipette Volume
  • Distribute
  • Consolidate


Variable Settings

  • Speed
  • Volume
  • Count
 


Other

  • Dispose

 

 

Applikációk

 

General Liquid Handling

The quickest way to save time in the lab. You can easily automate a range of basic liquid handling tasks on the OT-2 pipetting robot, including tube and microplate filling, serial dilution, cherry picking, normalization and sample pooling. Choose from a range of verified protocols in our Protocol Library, create your own, or ask our Applications Engineering team to create one for you.

PCR Setup

Another popular application for automation, especially if you’re prepping a lot of samples. You can use the OT-2 pipetting robot to combine samples and PCR mastermix in the reaction plate that will go into the thermocycler or qPCR machine.

Protein Purification

The most cost-effective way to automate small-scale protein purification, proteomics sample processing, and enzymatic and chemical lysis. We currently offer two approaches to automate on the OT-2 pipetting robot – dual flow chromatography-based automation, and magnetic bead-based automation.

 

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