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PCR, RT, PCR-Cloning

Experience improved PCR performance with GoTaq® DNA Polymerase products.
Amplify up to 30kb from genomic DNA using GoTaq® Long PCR Master Mix, a new system that allows easy setup of sensitive and efficient long PCR.
Discover sensitive, dependable qPCR and RT-qPCR kits for probe- or dye-based real-time PCR and multiplex qPCR.
You cannot walk by pGEM®-T, Promega's legendary T-vector cloning systems.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

GoTaq® G2 Hot Start Polymerase

Second generation GoTaq product—bound to a proprietary antibody to block activity below 70°C, increasing reaction specificity

GoTaq® G2 Hot Start Master Mixes

Ready-to-use mixes that contain all necessary components, including the 2nd-generation GoTaq G2 Hot Start Polymerase, for your PCR. Add template and go.

GoTaq® Hot Start Polymerase

Contains high-performance GoTaq® DNA polymerase bound to a proprietary antibody that blocks polymerase activity below 70°C and is restored during the initial denaturation step, increasing reaction specificity.

GoTaq® Hot Start Master Mixes

Simply add template, water and primers to the ready-to-use solution, and get the specificity and sensitivity of an antibody-based hot-start polymerase in either Green or Colorless Master Mixes for direct-to-gel analysis.

 

Routine PCR

Experience improved PCR performance with GoTaq® DNA Polymerase products.

GoTaq® G2 Flexi DNA Polymerase

The second generation of GoTaq® products with magnesium supplied separately, so you can optimize the Mg2+ concentration for your reactions.

GoTaq® G2 DNA Polymerase

Achieve the high-performing results you need with the second generation of GoTaq® DNA Polymerase supplied with both green and colorless reaction buffers containing magnesium.

GoTaq® G2 Green and Colorless Master Mixes

Ready-to-use green or colorless 2X master mix with all necessary components for robust, reliable PCR—contains second-generation GoTaq® G2 DNA Polymerase. Add template, primers and go!

 

 

RT-PCR

Easy, dependable and robust reverse transcriptases and RT-PCR kits for reverse transcription of full-length cDNA from abundant and rare mRNAs.

GoScript™ Reverse Transcription System

Produce multiple cDNAs using oligo(dT)15 or random primers during first-strand cDNA synthesis and/or divide into multiple second-strand cDNA syntheses and PCR amplifications with different gene-specific primers.

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

A cost-effective, quality reverse transcriptase for routine RT-PCR and cDNA synthesis.

AccessQuick™ RT-PCR System

Saves you four pipetting steps by combining several components into a master mix.

AMV Reverse Transcriptase

Great for RT-PCR templates with high secondary structure.

PCR Cloning

General PCR Cloning: pGEM®-T Vector Systems

Rapid and reliable system has no need for post-PCR modification. Cloning is complete in one hour at room temperature and easily releases the insert from the vector.

Direct Mammalian Expression: pTargeT™ Vector

Easily express cloned PCR products in mammalian cells using the powerful CMV promoter. Offers increased expression due to the SV40 late poly(A) sequence and origin of replication.

 

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