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SUN Bioscience

Sunbioscience Gri3D platek

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plate
Hydrogel-lel előre bevont, „ready-to-use” 3D sejttenyésztő, 96-well plate-k szferoidok és organoidok tenyésztéséhez és standardizálásához.

A microwell-ek külünböző pórusméretben elérhetőek többféle sejttípushoz/ mintához, valamint segítik a nagyszámú minták tesztelését például, gyógyszerhatóanyagok fejlesztésére.

Bővebb információt és részletes leírást talál a gyártó weboldalán:

Sunbioscience



ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

The global leading technology and endless possibilities for standardized organoid and spheroid cultures.

 

Since 2016, SUN bioscience has been revolutionizing Precision Health. Our mission is to empower personalized medicine by harnessing the potential of patient stem cells to identify the most effective drugs. At the heart of our groundbreaking efforts is our proprietary high-throughput 3D microwell platform, enabling the generation of standardized and reproducible organoids, without any effort.

SUN’s flagship technology Gri3D® overcomes the challenges in organoid culture: automation, robustness, scalability, and easy imaging – introducing a new era in drug development.

 

Gri3D® is a ready-to-use platform for high-throughput and reproducible organoid culture.

Based on an array of ultra-dense U-bottom shaped microwells in hydrogel, single organoids are robustly generated in each microcavity and grown in suspension-like culture without a solid extracellular matrix. With multiple configurations available, there is a microwell size that will fit your organoid model.

 

Key Features of Gri3D plates:

ü   Pre-Formed And Ready-To-Use.

Gri3D® uses the most advanced hydrogel components available. Our PEG-based hydrogels are pre-formed, attached to the bottom of your Gri3D® plate and require no prior knowledge in hydrogel handling.

 

ü   Extreme Aspect Ratios For Extreme Reliability.

Ultra-dense U-bottom microwell arrays in hydrogel with sidewalls thinner than a human hair. One step cell seeding, no cells resting on the outside.

 

ü   3D Imaging Has Just Become Easier.

Gri3D® is designed to make your experience for 3D imaging as easy as for 2D. Gri3D® microwells are formed in hydrogels consisting mostly of water, allowing for refractionless imaging. Also, all organoids are located in a single imaging plane, meaning less imaging time and less data storage required.

 

ü   Made To Order.

Gri3D® is the most versatile microwell system available. Find the right size and shape to fit your organoid system. Round microwells are available in a range from the size of a single cell up to 3mm to fit even the largest mini brain. Enquire about special sizes and shapes.

 

ü   One Hydrogel, A Thousand Possibilities.

Our PEG-based hydrogels are cellrepellent by nature, allowing precise cell aggregation from ten to multiplethousands of cells. But they also have a versatile chemistry to optimize your organoid protocols.

 

ü   Your Solution To 3D Culture Automation.

Gri3D® was designed to standardize organoids for screenings. Our custom plate design according to SBS industry standards, allows to change media continuously using robotic systems and fully without disturbing your organoids.

 

ü   Homogeneous.

Forget border effects. Gri3D® has special meniscus-altering features allowing homogeneous cell seeding over the entire microwell array.

 

ü   High-Content Imaging.

Have you ever dreamt of effortless immunostaining protocols? SUN bioscience teams up with IBIDI Germany to provide Gri3D® with imaging bottom, allowing to culture, stain and confocal image your organoids on one single plate.

 

ü   High-Throughput Cryosections.

Gri3D® microwell arrays are made of hydrogels that can be cryosectioned together with your large organoid models. All in one plane.

 

ü   Customize your order and visit Products - SUN bioscience

 

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Amennyiben segítségre van szüksége, vagy árajánlatot szeretne kérni, legyen szíves használja az ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK! gombot és munkatársunk hamarosan felveszi önnel a kapcsolatot!

 

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Műszaki specifikációk

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GRI3D PRODUCT FAQ:

 

What is the shelf-life of Gri3D® and how should I store them?

Upon receipt, please store the plates at 4°C where they are stable for at least 6 months.

 

Do I need any special equipment to use Gri3D®?

Gri3D® products are SLAS-standard and are compatible with standard laboratory equipment suchas multichannel pipets or high-content imagers.

 

Is Gri3D® automatable?

Yes. We have established automated organoid workflows including hydrogel equilibration, cell

seeding, medium exchange and compound exposure with liquid handlers.

 

What are the plate dimensions?

96 wellplate: 127.90 x 85.60 x 14.45 mm (l x w x h).

24 wellplate: 127.90 x 85.60 x 19.00 mm (l x w x h).

Technical drawings are available upon request at Bio-Science Ltd.

 

What are the recommended well volumes?

96 wellplate: 50 μl seeding chamber + 150 μl pipetting port.

24 wellplate: 200 μl seeding chamber + 800 μl pipetting port.

 

What material is Gri3D® made of?

Top plate: polystyrene.

Hydrogel microwell: poly-ethylene glycol (PEG).

Bottom: plastic (1 mm thick, polystyrene) or imaging bottom (0.18 mm thick, IBIDI polymer).

 

How do I choose between plastic or imaging bottom plates?

The choice of the plate bottom depends on your application needs. For high resolution imaging of immunostainings at 10X or higher magnification, imaging bottom (0.18 mm, IBIDI polymer) is preferred. For other types of assays, use plastic bottom.

 

What are the microwell sizes available?

Our Gri3D® plates come with various microwell sizes ranging from 100 μm to 1500 μm. Learn more about all the options by looking at our product catalogue.

- Standard microwell diameter (μm): 400 (121 μwells), 500 (73 μwells), 600 (55 μwells), 800

(31 μwells).

- Custom microwell diameter (μm): 100 (1677 μwells), 200 (511 μwells), 300 (211 μwells), 1000

(19 μwells), 1500 μm (7 μwells).

** the number of μwells per wells is given for a 96 wellplate.

To help you get started, we propose sample plates to test the different microwell sizes (only

available for standard options). These special plates contain 1 column filled with hydrogel for each of the standard microwell sizes (400 - 800 μm).

 

How do I choose microwell size?

The purpose of having different diameters of microwells is to accommodate different organoid

types and sizes. If your organoids are small or develop in a short time from low cell amounts,

smaller microwell diameters will allow you to get a higher microwell density per area, therefore resulting in more datapoints per well. If you plan to grow your organoids to for longer times or to larger sizes, you can opt for larger microwell sizes.

We suggest that you start with our sample plates that contain 1 column filled with hydrogel for

each of the standard microwell sizes (400 - 800 μm). This will enable you to determine the optimal microwell size for your application. If you can share the organoid model that you wish to grow, our applications team will provide you with the best microwell size range to test.

 

What is the difference between standard and custom products?

Gri3D® standard plates come with microwell diameter sizes ranging from 400 to 800 μm. It can be ordered in 2 formats: 96 wells (full 96 wellplate) or 24 wells (in 96 wellplate).

Gri3D® custom plates come with extended microwell diameter sizes (from 100 to 1500 μm). It can be ordered on 96 wellplate or 24 wellplate formats in a column-basis, from 1-column to a full plate. Only one microwell size can be chosen per plate. Production of custom products is upon demand with 5% extra fee and increased delivery time. The order is only possible by requesting a quote to Bio-Science Ltd.

Applikációk

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