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Beckman Coulter

Optima AUC

Beckman Coulter delivered the first AUC sample characterization tool to the scientific community powering discoveries. The tradition continues into the 21st century with the new Optima AUC system.
This latest offering is the most robust technology for providing protein molecular weight in basic protein research and quantification of aggregation levels for academic and biopharma research.
Analytical ultracentrifugation is the most versatile, rigorous and accurate means for determining the molecular weight, hydrodynamic and thermodynamic properties of a protein or other macromolecule.
Currently, there is no other technique capable of providing the same range of information with a comparable level of precision and accuracy.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

Optima AUC Features

The Optima™ AUC combines the power of a centrifuge to provide particle sedimentation with the functionality of optical modules to detect the sedimentation over time. It provides the data you need even faster, so you can make new discoveries in less time.

Summary of features and benefits:

  • Enables sample recovery
  • Avoids dilution effects
  • Analyzes samples in a matrix-free environment
  • Has minimal buffer constraints
  • Enables detection at low concentrations
  • Requires low sample volume (0.1 mL minimum)
  • Delivers high-throughput analysis – up to 7 samples in a single run
  • Requires no standards – relies upon first principles of thermodynamics

Versatility

  • Analyzes a wide array of particles in native, matrix-free conditions, including:
  • Proteins
  • Nanoparticles
  • Peptides
  • Polymers
  • Micelles
  • Liposomes
  • Extracellular vesicles
  • Drug conjugates
  • Viral payload

Provides data which can answer more critical questions than any other comparable technique, including:

  • Shape
  • Mass
  • Diameter
  • Stoichiometry
  • Heterogeneity
  • Association
  • Aggregation
  • Purity
  • Formulation

Enables precise analysis of complex systems at discrete wavelengths (up to 20) in minimal time.

Time Savings

• Generates faster results as acquisition time is no longer additive.
• Provides even more scans with the use of an An-50 Ti 8-hole rotor.

Ease of Use

Optima AUC Ease of Use

  • Modular design provides absorbance and interference optical systems—with the option to install up to 3 independent, simultaneous detection systems (see photo).
  • User-friendly touchscreen display indicates intuitive progression of experimental design.
  • Remote monitoring capabilities let you set up, monitor and extract data from virtually any location.
  • Compatibility with ProteomeLab XL cells and rotors—and widely used analytical software (e.g., UltraScan, SEDFIT/SEDPHAT, SEDANAL, DCDT+)—helps simplify the upgrade process.

Data Quality

  • Delivers cleaner data by providing higher radial resolution and higher signal-to-noise ratio.
  • Delivers higher precision, reproducibility and accuracy than the ProteomeLab XL.

Optima AUC Live Data Acquisition

 

Műszaki specifikációk

 

Product Specifications

Acceleration 5-400 rpm/second (above 500 rpm), 400 max acceleration
Deceleration 5-400 rpm/second (above 500 rpm), 400 (max deceleration)
Speed Control ± actual rotor speed, ± 2 rpm at steady state
Timed Run 1 to 999 hrs, 59 mins (including hold runs)
Max Capacity Volume Range 0.10 - 0.44 mL
Operating System(s) LINUX
Speed Display 1,000 to rated speed in 100 rpm increments
Temperature Control 0.50 °C of set temperature after equilibration
Display 15.3" high-resolution color touch screen
Temperature Range(s) Ambient temperature range: 10 to 30°C, Set temp: 7-40° C (1°C increments at > 25-30°C ambient temp); 4-40°C (1°C increments at <25°C ambient temp)

 

Applikációk

 

Analyzes a wide array of particles in native, matrix-free conditions, including:

  • Proteins
  • Nanoparticles
  • Peptides
  • Polymers
  • Micelles
  • Liposomes
  • Extracellular vesicles
  • Drug conjugates
  • Viral payload

Provides data which can answer more critical questions than any other comparable technique, including:

  • Shape
  • Mass
  • Diameter
  • Stoichiometry
  • Heterogeneity
  • Association
  • Aggregation
  • Purity
  • Formulation

 

 

Tartozékok, kiszerelések

 

INSTRUMENTS & COMPONENTS

Ordering Information Component Part No. Description

Optima AUC - Absorbance Only B86438 Hardware only with Absorbance optics

Optima AUC - ABS/INT B86437 Hardware only with Absorbance + Interference optics

Optima AUC – ABS Bundle + An-60 C00707 Optima AUC (A) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-60

Optima AUC – ABS/ INT Bundle + An-60 C00708 Optima AUC (A/I) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-60

Optima AUC – ABS Bundle + An-50 C00709 Optima AUC (A) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-50

Optima AUC – ABS/ INT Bundle + An-50 C00710 Optima AUC (A/I) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-50

An-60 rotor 361964 4-hole rotor rated to 60,000 rpm

An-50 rotor 363782 8 hole rotor rated to 50,000 rpm

2-sector charcoal Quartz 392772 SV cell with charcoal flow-through centerpiece and quartz windows

Torque stand Assembly 361318 Required to torque cells to prevent sample leaking

Counterbalance Kit 360219 1 counterbalance with mask + weights

Accessory Kit 392777 6 brass housing plugs

Cell housing kit 334606 2 window assemblies + cell housing

 

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