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Promega

Klónozás,markerek,vektorok

Here you find everything you may ever need for cloning: competent cells, restriction enzymes, markers and a variety of vectors.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Cloning Tools and Competent Cells

Cloning enzymes, DNA modifying enzymes, subcloning vectors, competent cells and mutagenesis tools.

 

Molecular Weight Markers

DNA ladders, RNA markers and protein molecular weight markers.

 

PCR Cloning

Product details and technical resources for T-vector cloning: The easy way to clone and express PCR products.

General PCR Cloning: pGEM®-T Vector Systems

Rapid and reliable system has no need for post-PCR modification. Cloning is complete in one hour at room temperature and easily releases the insert from the vector.

Direct Mammalian Expression: pTargeT™ Vector

Easily express cloned PCR products in mammalian cells using the powerful CMV promoter. Offers increased expression due to the SV40 late poly(A) sequence and origin of replication.

 

Restriction Enzymes

High quality, high activity restriction enzymes for all your research needs.

Restriction Enzyme Buffer Compatibility

Looking for compatible buffers for a double digest? The reference chart linked below provides a convenient method to find the activity of Promega enzymes in our 4-Core buffer system.

Thermosensitive Alkaline Phosphatase

Prevent recircularization and religation of your linearized cloning vector DNA during ligation without additional DNA cleanup steps.

 

Bacterial Expression Vectors

Vectors designed for expression of proteins in E. coli from the T7 RNA Polymerase promoter, with or without the addition of purification tags.

 

Cell-Free Expression Vectors

Vectors designed for use with Promega cell-free expression systems, including E.coli, rabbit reticulocyte lysate, wheat germ extract, and insect cell extract-based systems.

 

Mammalian Expression Vectors

Includes the multi-purpose Flexi® Vectors, the pCMVTNT™ Vector, the pTargeT™ T-Vector, and systems for regulated mammalian expression.

 

Reporter Vectors

Reporter vectors for promoter analysis, nuclear receptor studies, protein interaction analysis and signaling pathway studies.

NanoLuc® Luciferase Reporters

State-of-the-art reporter technology offering unrivaled sensitivity and versatility in a very small luciferase. Find the pNL reporter that is right for your application.

Coincidence Reporter Vectors

Express firefly and NanoLuc® luciferases from the same transcript. Generate two unfused luciferase enzymes with distinct compound interaction profiles, allowing convenient identification of true and false hits in HTS compound screening.

pGL4 Luciferase Reporter Vectors

Optimized luciferase reporter vectors with both firefly and Renilla luciferases in a variety of configurations. Now also available with response elements to study pathways of interest.

miRNA Target Expression Vector

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2).

 

RNAi Vectors

Vectors for expressing shRNAs in vivo, and measuring the RNAi effect.

 

Subcloning and Transcription Vectors

Routine, and not so routine, vectors for your everyday subcloning needs. Blue/white screening, various RNA polymerase promoters, and a range of choices for insert subcloning and recombinant selection.

 

T Vectors

Vectors designed for cloning PCR products and for expression of cloned PCR products in mammalian cells.

General PCR Cloning: pGEM®-T Vector Systems

Rapid and reliable system has no need for post-PCR modification. Cloning is complete in one hour at room temperature and easily releases the insert from the vector.

Direct Mammalian Expression: pTargeT™ Vector

Easily express cloned PCR products in mammalian cells using the powerful CMV promoter. Offers increased expression due to the SV40 late poly(A) sequence and origin of replication.

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