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SpectraMax iD5e Multi-Mode Microplate Reader

The SpectraMax® iD5e microplate reader offers advanced detection capabilities with its five
detection modes: absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF),
and tunable fluorescence polarization (FP). Supported by hybrid optics, a touchscreen interface,
and temperature control, this reader provides flexibility and precision for complex assays.
Optional upgrades include bottom-read luminescence, TR-FRET (including HTRF®), and western
blot detection. For live cell assays and microbial growth experiments, environmental controls for
CO2/O2 and advanced shaking are also available.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Leírás

Superior hybrid optical system

The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.

Live cell assays

Advanced shaking and user-installable gas mixer (CO₂/O₂) that support ideal environments for cell-based and microbial growth experiments are available as optional upgrades.

Automatic filter identification

Automatically identify filter sets using NFC functionality, simplifying workflows and ensuring accuracy and reliability.

Flexible temperature control

Precise and intuitive temperature regulation—from 5°C above ambient conditions up to 66 °C—empowering your lab to perform a wide range of temperature-sensitive assays with confidence.

Read height optimization (Z height)

Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.

Microvolume & cuvette options

The SpectraDrop Micro-Volume Microplate for low-volume (2 to 4 μL) and SpectraCuvette™ Adapter Plate for cuvette-based applications eliminate the need for additional instrumentation.

Personalized touchscreen control

The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

Western blot capable

The optional enhanced time-resolved fluorescence (TRF) detection module is optimal for quantitating as little as femtogram protein samples, providing improved sensitivity, extended signal stability and versatility to support a wide range of applications.

Műszaki specifikációk

General specifications	SpectraMax iD3s	SpectraMax iD5e
Read modes	Absorbance Fluorescence (top/bottom read) Luminescence (top read)	Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top/bottom read) HTRF (top/bottom read) HTRF
Wavelength ranges	Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm	Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm TRF Ex: 350 nm TRF Em: 450 - 750 nm FP: 300 - 750 nm
Wavelength selection	Monochromator tunable in 1-nm increments	Hybrid filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity	< ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV	< ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Absorbance photometric precision/repeatability	< ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV	< ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Sensitivity top read optimized	FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM	FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well
Light source(s)	High power xenon flash lamp	High power xenon flash lamp
Detector(s)	Silicon photodiode Ultra-cooled photomultiplier tube	Silicon photodiode Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc)	Endpoint Kinetic Spectrum scan Well scan	Endpoint Kinetic Spectrum scan Well scan
Read times (kinetic interval for 96-well plate)*	Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds	Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds TRF: 25 seconds FP: 35 seconds
Plate shaking	Linear Orbital Double orbital	Linear Orbital Double orbital
Injectors	dual injector, optional	dual injector, optional
Western blot	not available	with optional cartridge
Stand-alone touchscreen operation	yes	yes
Robotics / automation	compatible	compatible
Plate type(s)	6-384 well plates	6-384 well plates
Temperature control	Ambient + 5°C to 66°C	Ambient + 5°C to 66°C
Dimensions	H 40.1 cm x W 53.2 cm x D 59.8 cm	H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools	Absorbance validation plate Fluorescence validation plate Luminescence validation plate SoftMax® Pro GxP Software	Absorbance validation plate Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software

Applikációk

The SpectraMax iD5e is a hybrid monochromator- and filter-based, five-mode microplate reader that allows you to expand the boundaries of your research to include a wide variety of applications.