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BioLector XT

GET MO2RE DATA. NOW. With or without O2
The BioLector XT high-throughput microbioreactor enables real-time evaluation of biomass, pH, dissolved oxygen in the liquid phase (DO), fluorescence and other key cultivation parameters for aerobes and anaerobes— to quickly provide deep insights into your bioprocess development.

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with non-invasive, pre-calibrated optical sensors.

Disposable 48-well MTPs enable online measurement of key cultivation parameters while an optional microfluidic module supports simultaneous, well-individual pH control and feeding.

This patented microfluidic technology eliminates manual liquid handling—no tubing/pipetting required, as everything is part of the gamma-radiated ready-to-use plate.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Leírás

BioLector XT Microbioreactor Features

INNOVATIVE NEW GASSING LID	OPTIONAL MICROFLUIDIC MODULE	“PLUG-AND-PLAY” PLATE DESIGN	INTELLIGENT BIOLECTION SOFTWARE
Enables fed-batch experiments under anaerobic conditions	Unleashes full potential of the BioLector XT	Online data out of 48/32 parallel cultivations	Intuitive user interface supports multi-user environments
Gassing with O22within a range of 1% - 100% and with CO2 within 0% - 12 %	Complements online monitoring function with well-specific pH regulation/feeding	Customizable feeding strategies (batch, fed-batch)*	Free programming of all control parameters
Reduces gas consumption to a few mL/minute	Enables use of 2 feed lines per cultivation well	Control of pH on-the-plate with pre-calibrated optical sensors*	Open system enables live data downloads
Optional humidification of gases reduces evaporation	Microvalves allot liquids at nanoliter-scale	Small working volume (800 – 2400 μL)	Fast processor ensures rapid download of experiment data
		*Functionality requires optional microfluidic module

Additional features include:

Capability to fully customize cultivation protocols to cover a broader spectrum of applications
Enables free combination of different feeding and pH control strategies over one cultivation run
Updated BioLection software provides an intuitive user interface designed for multi-user environments
New gassing lid reduces gas consumption and acts as an air-tight anaerobic chamber that can be used with the microfluidic module, eliminating the need for anaerobic tents.

Műszaki specifikációk

BioLector XT adatlap

System Performance

32/48 parallel microreactions, optionally 16 reservoir wells
Working volume: 800 – 2400 μL
Pre-calibrated optical sensors for online pH and DO measurement
Well-individual pH control and feeding
Fully flexible feeding options: constant, linear, exponential or signal-triggered, with any combinations possible
Broad range of kLa values (30 – 600 h-1)
Continuous gas exchange and oxygen supply
Strictly anaerobic cultivations possible, optionally with active pH control and feeding
Equal power input into each reactor
Defined engineering parameters and scalability
Controlled gas atmosphere (CO2, O2)
Temperature control (8 °C below ambient temperature to 50 °C) with active water cooling

"Plug and Play" Microtiter Plate Design

Real-time kinetics out of 48/32 parallel cultivations
Fully customizable and freely combinable actively regulated feeding strategies (batch, fed-batch, bolus, continuous)*
Control of pH on-the-plate with precalibrated optical sensors*
Flexible process control of pH, shaking, temperature and gassing
Strictly anaerobic cultivations with feeding and pH control*
Cultivation of highly oxygen-demanding strains with up to 100 % O2
DO and signal-triggered feeding*
Low pH measurements and control* available in the range of 4-6
High-throughput and easy automation
Broad range for biomass detection (equivalent to up to 250 OD600, 50 g/L CDW, measured with E. coli)
Online biomass measurement requires no dilution
Small working volume (800 – 2400 μL)
No edge effects
Continuous shaking operation (no artifacts)
Defined mass transfer conditions
Reliable scale-up to bench-top fermenters
A valuable tool for PAT and QbD

*Note: functionality requires the optional microfluidic module

Applikációk

MO2RE flexibility for more applications

Feeding strategy development
Feeding rate optimization
Media screening and optimization
Cultivation parameter optimization
pH profiling • Strictly anaerobic and microaerophilic cultivations in batch and fed-batch mode
High-oxygen (up to 100 %) and high-carbon dioxide (up to 12 %) cultivations
Cell line and strain screening
Synthetic and systems biology
Statistical design of experiments (DoE)
Growth characterization
High-throughput protein expression
Enzyme and cell activity tests
Functional genomics
Proteomic studies
Inhibition and toxicity tests
Quality control

Online Measurement / Online Control

MO2RE control & more data for deeper insights

Online Measurement

Biomass concentration
pH value
Dissolved oxygen (DO)
NAD(P)H and Riboflavins
Fluorescent molecules (e.g., GFP, YFP, DsRed)
Shaking speed
Temperature
O2 in head space atmosphere
CO2 in head space atmosphere

Online Control

pH value (well-specific)
Feeding (well-specific)
Shaking speed
Temperature
Gas flow
O2 in head space atmosphere
CO2 in head space atmosphere

Tartozékok, kiszerelések

Optional Microfluidic Module helps BioLector XT microbioreactor do even MO2RE

Unleashes the full potential of the BioLector XT microbioreactor
Complements online monitoring function with well-specific pH regulation and feeding
Enables use of 2 reservoir wells per 4 cultivation wells—with either 2 pH-adjusting solutions, 2 feed solutions or 1 of each
Liquids allotted in nanoliter-scale through microvalves

Videók, ismertetők

BioLector XT ismertető

BioLector XT adatlap